建立了固相萃取-反相高效液相色譜法測(cè)定隱形眼鏡護(hù)理液中聚胺丙基雙胍的方法。

固相萃取-反相高效液相色譜法測(cè)定隱形眼鏡護(hù)理液中聚胺丙基雙胍  
   用Spe-ed WCX固相萃取柱(500 mg/3 mL)將樣品濃縮富集后,以Shiseido C18色譜柱(150 mm×4.6 mm,5μm)為分離柱,以pH 4.0的乙-腈20 mmol.L-1乙酸銨(體積比16比84)為流動(dòng)相,檢測(cè)波長(zhǎng)為235 nm,7 min內(nèi)測(cè)定樣品中聚胺丙基雙胍。方法檢出限(S/N=3)為0.046 mg.L-1,相對(duì)標(biāo)準(zhǔn)偏差為3.3%(n=7),線性范圍為100~800 mg.L-1,加標(biāo)回收率93.5%~110.9%。
    SPE-RHPLC was applied to the determination of polyhexamethylenebiguanide(PHMBG) in lens cleaning-disinfectant solution.PHMBG was enriched on the Spe-ed WCX solid phase extraction column and separated on the shiseido C18 column.A mixed solution of acetonitrile and 20 mmol·L-1 ammonium acetate(mixed at a ratio of 16 to 84) at pH 4.0 was used as mobile phase.UV-detection at 235 nm was adopted in the determination.Linear relationship between values of peak area and concentration of PHMBG was obtained in the ran...